Discovery begins with Sample Preparation
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Sample processing is a signficant bottleneck in genomics and proteomics research when dealing with tissues and cells. A novel technology from Pressure Biosciences (PBI) allows the safe, rapid and reproducible extraction of DNA, RNA, small molecules and proteins from a wide variety of cells and tissues.
By cycing rapidly cycling pressure between ambient and ultra high levels (up to 35,000 psi) at controlled temperatures, PBI's platform is able to faciliate the lysis of tissue and cells. Using PBI's sample preparation reagents, proteins, lipids and nucleic acids can by extracted simutaneously without the need for detergents which can often interfere with downstream analysis techniques.
By increasing protein recovery and providing improved reproducibility compared to traditional lysis methods, PBI's technology can enhance your chances of finding robust novel biomarkers from difficult-to-process animal, plant, virus and bacterial samples.
Certain types of samples cannot be efficiently extracted by any single sample preparation method; therefore, Pressure Biosciences have developed the CPT Shredder which improves sample homgenization and subsequent extraction of tough, fibrous, and other difficult to distrupt samples. Following general mechanical disruptio with the PCT Shredder, extraction can then be performed by PCT.
In addition to sample lysis, Pressure Biosciences have also developed a number of applications to increase the efficiency and reduce the time taken from proteolytic digests of proteins prior to mass spectroscopy.
A range of other examples where PCT has been used in genomic, proteomic and metabolmic applications are available on our website under Application Notes and Third Party Publications (www.pressurebiosciences.com).